Journal: Frontiers in Veterinary Science
Article Title: Androgen receptor activation promotes tumor progression in canine and human triple negative breast cancer cell lines
doi: 10.3389/fvets.2025.1677830
Figure Lengend Snippet: Effects of DHT and E2 administration on IPC-366 CNT and siAR cells. (A) AR silencing in IPC-366 cells. AR, p-AR and ARV7 expressions in IPC-366 control with and without E2 and DHT administration. Bars represent protein intensity respect to GADPH expression and data was represented as percentage respect to control (CNT) group. (B) Graphs represent percentage of cell viability of siCNT and siAR cells (left), and after DHT or E2 administration. (C) Steroid hormone concentrations secreted by siCNT and siAR cells with or without DHT and E2. Bars represent ± SD of DHEA, P4 (upper), E1, E2 (middle), T, A4, and DHT (lower) concentrations. *Denoted significant differences ( p < 0.05) between siCNT and siAR. (D) EGFR, STAT3, Src, Erβ, Akt, ERK1/2 and Ras protein expression in control and siAR cells and with E2 or DHT administration.
Article Snippet: Anti-AR (PA1-37079; 1/500); anti-p-AR (PA5-106115;1/1000); anti-ERβ (51–7,700; 1/500); anti-EGFR (PA1-1110; 1/1000); Anti-STAT3 (MA1-13042; 1/2000); Anti-Ras (PA5-87037; 1/1000); Anti-Src (44–655; 1/1000); Anti-Akt (PA5-104548; 1/1000); Anti-ERK1/2 (MA5-15134; 1/1000) (ThermoFisher Scientific, Massachusetts, USA); ARV7 (68,492; 1/500) (Cell Signaling Technology, Danvers, MA, USA), and GADPH (used as loading control) (HRP-60004; 1/10,000) (Proteintech, Planegg-Martinsried Germany), were used as primary antibodies.
Techniques: Control, Expressing